1. Concentration of Samples (ng/μL)

+

2. Sizes of Samples (kb)


3. Number of enzymes


4. Desired total volume (µL)



Recipes for each sample (μL)

If a recipe has a negative Milli Q value, you do not have enough DNA to perform the digest with the amount of DNA you put in Step 2.

DNA Template
Enzyme (each)
Buffer
Milli Q Water